P‐43 IgG antibodies against sarcoptic mite antigens in dogs cross‐reacting with house dust and storage mite antigens
Identifieur interne : 008A25 ( Main/Exploration ); précédent : 008A24; suivant : 008A26P‐43 IgG antibodies against sarcoptic mite antigens in dogs cross‐reacting with house dust and storage mite antigens
Auteurs : B. Bigler [Suisse] ; F. Virchow [Suisse]Source :
- Veterinary Dermatology [ 0959-4493 ] ; 2004-08.
Abstract
Sera from dogs suffering from scabies were used to evaluate possible antigenic cross‐reactivity with proteins from house dust or storage mites. Polyacrylamide gel electrophoresis on gradient gels was used to create size‐dependent protein bands of Sarcoptes scabiei ssp. vulpis, Dermatophagoides farinae, D. pteronyssinus, Acarus siro, Lepidoglyphus destructor and Tyrophagus putrescentiae. Anti‐canine IgG antibodies conjugated with alkaline phosphatase were used for immunostaining. Different patterns for Sarcoptes could be seen with strong bands repetitively observed of approximately 22, 112, 116, 132 and 200 kD in size. The band at approximately 22 kD seems likely to have cross‐reactivity with a protein of the same size in A. siro. The one at approximately 200 kD might share antigenic activity with bands of D. farinae and, to a lesser extent, D. pteronyssinus. Funding: Laupeneck AG.
Url:
DOI: 10.1111/j.1365-3164.2004.00414_43.x
Affiliations:
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IgG antibodies against sarcoptic mite antigens in dogs cross‐reacting with house dust and storage mite antigens</title><author><name sortKey="Bigler, B" sort="Bigler, B" uniqKey="Bigler B" first="B." last="Bigler">B. Bigler</name><affiliation wicri:level="3"><country xml:lang="fr">Suisse</country><wicri:regionArea>Laupeneck AG, Labor, Bern</wicri:regionArea><placeName><settlement type="city">Berne</settlement><region type="région" nuts="3">Canton de Berne</region></placeName></affiliation></author><author><name sortKey="Virchow, F" sort="Virchow, F" uniqKey="Virchow F" first="F." last="Virchow">F. Virchow</name><affiliation wicri:level="3"><country xml:lang="fr">Suisse</country><wicri:regionArea>Laupeneck AG, Labor, Bern</wicri:regionArea><placeName><settlement type="city">Berne</settlement><region type="région" nuts="3">Canton de Berne</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Veterinary Dermatology</title><title level="j" type="alt">VETERINARY DERMATOLOGY</title><idno type="ISSN">0959-4493</idno><idno type="eISSN">1365-3164</idno><imprint><biblScope unit="vol">15</biblScope><biblScope unit="page" from="54">54</biblScope><biblScope unit="page" to="54">54</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2004-08">2004-08</date></imprint><idno type="ISSN">0959-4493</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0959-4493</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Sera from dogs suffering from scabies were used to evaluate possible antigenic cross‐reactivity with proteins from house dust or storage mites. Polyacrylamide gel electrophoresis on gradient gels was used to create size‐dependent protein bands of Sarcoptes scabiei ssp. vulpis, Dermatophagoides farinae, D. pteronyssinus, Acarus siro, Lepidoglyphus destructor and Tyrophagus putrescentiae. Anti‐canine IgG antibodies conjugated with alkaline phosphatase were used for immunostaining. Different patterns for Sarcoptes could be seen with strong bands repetitively observed of approximately 22, 112, 116, 132 and 200 kD in size. The band at approximately 22 kD seems likely to have cross‐reactivity with a protein of the same size in A. siro. The one at approximately 200 kD might share antigenic activity with bands of D. farinae and, to a lesser extent, D. pteronyssinus. Funding: Laupeneck AG.</div></front></TEI><affiliations><list><country><li>Suisse</li></country><region><li>Canton de Berne</li></region><settlement><li>Berne</li></settlement></list><tree><country name="Suisse"><region name="Canton de Berne"><name sortKey="Bigler, B" sort="Bigler, B" uniqKey="Bigler B" first="B." last="Bigler">B. Bigler</name></region><name sortKey="Virchow, F" sort="Virchow, F" uniqKey="Virchow F" first="F." last="Virchow">F. Virchow</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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